Database test

Proteomics Center at Vilnius University Institute of Biochemistry is dedicated to perform proteomics analysis starting from sample preparation by 1D and 2D electrophoresis as well as chromatography and subsequent mass spectrometry protein identification and quantitation. Proteomics center is equip with MALDI TOF-TOF mass spectrometer used mainly for protein identification from gel samples, Q-trap 4000 hybrid triple-quadruple and linear trap mass spectrometer for protein posttranslational modification analysis and recently acquired advanced Higher Definition Mass Spectrometer Synapt G2 for label-free high throughput differential proteome analysis. Synapt G2 HDMS mass spectrometer consists of a sample preparation component chromatograph nanoACQUITY UPLC system, Waters directly connected to the mass spectrometer and software for data analysis.

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The equipment is used for magnetronic deposition of metals (chromium and gold) on various film thickness under pressure control. Chamber volume is 75 liters; Sampler holder - 30 cm in diameter; Magnetron target size (diameter) 50.8 mm wit the deposition chimney; Heating up to 350 ˚C; Basic chamber pressure 2x10-7 Torr.

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The equipment is used to study protein-ligand association, interaction of proteins (peptides) with phospholipid membranes and adsorbtion of biomolecules. Baseline noise < 0.0001 degeree. Refractive index interval from 1.30 to 1.55, Refractive index resolution 10-6; Sensor material - BK-7; Dynamic range 4 degrees.

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The equipment is used to cover various surfaces by the rotating thin layer coating method. Samples size between 3 mm to  150 mm in diameter. Speed maximum 12000 rpm.

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The equipment is used to produce nanometric layers from amphiphilic molecules. Bath size 50x190 mm, Langmuir subphase volume 37 mm. Surface pressure measurements and controls is automated.

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The equipment is sed to evaluate hydrophilicity / hydrophobicity of surfaces. Contact angle measurements in  1-180 degree range. Angle resolution 0.1 degree. Positioning - mechanical.

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The system is used to produce micropipettes and capillaries with the tips of up to 0.3 mm thick and 6-10 mm long. Capillary radius up to 0.3 mm, accurate temperature control.

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The atomic force microscope is used for nanometric analysis of biomolecule and cell surfaces, for single-biomolecule and single-cell manipulations and to study their properties. Atomic force microscope with moving tip equiped with noise reducing chamber and electrochemical module. Top view system with 1.6 micrometer resolution. Fully automated camera. Sampler holder with vacuum pump. Scaner parameters: minimal scaning (XY) area 90 µm, Z range from 10 µm. Available measurement methods: tapping mode, contact mode. Imaging:  phase imaging, lateral force microscopy, force modulating, electrical field microscopy, magnetic force microscopy, piezoresponse force microscopy.

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The microscope is used for spectroscopic measurements. Raman detector Newton DU971P-UVB, spectral range 200 nm - 1100 nm; quantum efficiency: 200-400 nm  ≥30%; 400-500 nm ≥50%; 500-700 nm ≥80%; dark current 0,0002 e-/pixel/sec; pixel size 16 x 16 mm. UV objective 15x, 40x, flow cell, temperature controller, LTS420, T95, Linkam Scientific Instruments.

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The laser is used in a resonant Raman spectroscopy with different impulse laser wavelenght. Tuning range 1) 200-210 nm; 2) 210-260 nm; 3) 310-400 nm; 4) 410-650 nm 5) 740-1200 nm 6) 355 nm. Output power: 205 nm:  ≥2 mW; 210-260 nm: ≥20 mW; 310-400 nm: ≥20 mW; 410-650 nm:  ≥70 mW; 740-1000 nm: ≥100 mW; 355 nm: ≥2000 mW. Pulse duration 3-6 ns, beam divergence ≤4 mrad@450 nm.

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