Course Database test

The atomic force microscope is used for nanometric analysis of biomolecule and cell surfaces, for single-biomolecule and single-cell manipulations and to study their properties. Atomic force microscope with moving tip equiped with noise reducing chamber and electrochemical module. Top view system with 1.6 micrometer resolution. Fully automated camera. Sampler holder with vacuum pump. Scaner parameters: minimal scaning (XY) area 90 µm, Z range from 10 µm. Available measurement methods: tapping mode, contact mode. Imaging:  phase imaging, lateral force microscopy, force modulating, electrical field microscopy, magnetic force microscopy, piezoresponse force microscopy.

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The microscope is used for spectroscopic measurements. Raman detector Newton DU971P-UVB, spectral range 200 nm - 1100 nm; quantum efficiency: 200-400 nm  ≥30%; 400-500 nm ≥50%; 500-700 nm ≥80%; dark current 0,0002 e-/pixel/sec; pixel size 16 x 16 mm. UV objective 15x, 40x, flow cell, temperature controller, LTS420, T95, Linkam Scientific Instruments.

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The laser is used in a resonant Raman spectroscopy with different impulse laser wavelenght. Tuning range 1) 200-210 nm; 2) 210-260 nm; 3) 310-400 nm; 4) 410-650 nm 5) 740-1200 nm 6) 355 nm. Output power: 205 nm:  ≥2 mW; 210-260 nm: ≥20 mW; 310-400 nm: ≥20 mW; 410-650 nm:  ≥70 mW; 740-1000 nm: ≥100 mW; 355 nm: ≥2000 mW. Pulse duration 3-6 ns, beam divergence ≤4 mrad@450 nm.

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The laser is used for Raman spectroscopic measurements. Wavelength 266 nm, spectral linewidth  ≤0.3 MHz, output power 100 mW.

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The laser is used for Raman spectroscopic measurements. Wavelength 405 nm, spectral linewidth  ≤200 MHz, output power 40 mW.

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The laser is used for Raman spectroscopic measurements. Wavelength 457 nm, spectral linewidth  ≤1 MHz, output power 50 mW.

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The laser is used for Raman spectroscopic measurements. Wavelength 532 nm, spectral linewidth ≤1 MHz,  output power 150 mW.

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The Applied Biosystems 3130xl Genetic Analyzer 16-capillary automated DNA sequencer that yields 700 to 1000 bases per template. It performs cycle sequencing reactions using fluorescent dye ter­minators ABI Big Dye® Terminator v3.1 on any kind of DNA (plasmid, phage or PCR product) provided by the users.

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The device performs an initial effective characterization analysis of biomolecules and synthetic organic compounds by chromatographic and capillary electrophoresis. 

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Microbial fermentation bioreactor (Biostat C plus with 30L working volume) with  continuous flow centrifuge (CEPA Z41) and steam generator

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