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Upright microscope Zeiss Axio Imager.Z2 with confocal unit LSM 700. For fluorescence detection miscroscope dispose two PMT detectors, for transmission light T-PMT detector. Lasers: 405, 488, 555 nm. Detectors: 2 x PMT detectors, 1 x T-PMT detector. Objectives: 10x/0.3 EC Plan-Neofluar; 20x/0.4 Korr LD Plan-Neofluar; 40x/1.2 W Korr C Apochromat; 40x/1.0 DIC VIS IR; 63x/1.4 OIL DIC Plan-Apochromat Software: ZEN black. 

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.

Inverted microscope Zeiss Axio Observer.Z1 with laser scanning unit LSM 780. Microscope disposes solid state lasers - 405, 561 and 633 nm and argon laser - 455, 488, 514 nm. For detection of fluorescence signal in confocal mode there are two PMT detectors, 32 channel spectral GaAsP detector and Airyscan detector and T-PMT for transmission light detection. Microscope is also equipped with CCD camera AxioCam MRm. Lasers: 405, 458, 488, 514, 561, 640 nm. Detectors: 2 x PMT detectors, 1 x GaAsp PMT spectral detector, 32-channels Airyscan detector, T-PMT detector. Camera: Axiocam MRm, CCD sensor, 1388 x 1040 pixels, pixel size 6.45 x 6.45 um. Objectives: 10x/0.3 M27 EC Plan-Neofluar; 20x/0.5 M27 EC Plan-Neofluar; 25x/0.8 M27 LD LCI Plan-Apochromat W, Oil, Glycerol korr; 40x/1.2 M27 W Korr C-Apochromat; 63x/1.3 M27 Imm korr LCI Plan-Neofluar; 63x/1.4 M27 OIL DIC Plan-Apochromat Software: ZEN black. 

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.

Inverted microscope Zeiss Axio Observer.Z1 with confocal unit LSM 800. Microscope is equipped with four solid state lasers - 405, 488, 561, 640nm. For detection of fluorescent light in confocal mode there are three GaAsP PMT detectors and T-PMT detector for transmission light detection.For acquisition in widefield mode there is AxioCam 503 CCD camera. Lasers: 405, 488, 561, 640 nm. Detectors: 3 x GaAsp PMT, 1 x T- PMT detector. Filters for fluorescence: DAPI, GFP, Cy3, Cy5. Camera: Axiocam 503, CCD sensor, 1936 x 1460 pixels, pixel size 4.54 x 4.54 um. Objectives: 10x/0.3 – EC Plan-Neofluar; 25x/0.8 – LCI Plan-Neofluar; 40x/1.3 – Plan-Apochromat; 63x/1.2 – C-Apochromat; 63x/1.4 – Plan-Apochromat. Software: ZEN blue. 

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.

Thermo Scientific Talos Arctica – mid-range transmission electron microscope operating at 40 - 200 kV equipped with Schottky field emission gun (XFEG) providing highly coherent electron beam with ultimate stability. The microscope is equipped with Thermo Scientific Falcon3 EC direct electron detector and Ceta camera. Automated collection data for single particle analysis and cryo-electron tomography data can be performed with EPU or SerialEM software, respectively. The microscope is well suited for automated acquisition of high-quality cryoEM data, efficient screening of cryoEM samples, and 3D electron diffraction tomography

Modern electron microscopy in structural biology on the cellular and molecular level is performed by cryo-electron microscopy and cryo-electron tomography. Cryo-electron tomography (cryo-ET) is the only method to address pleiomorphic structures like cells and organelles in a close to native state, while cryo-electron microscopy (cryo-EM) is applied to study single particles, primarily larger macromolecular complexes, which have been isolated and purified by biochemical methods. Both methodologies provide information on the cellular and molecular level and are therefore ideal for in-depth structural-functional analysis in combination with state of the art biochemical characterisation.

Conventional cryoEM microscope FEI F20 (200 kV) equipped with a CCD camera and a dual beam FIB/SEM instrument (Versa3D) for micromachining of thin lamellas of vitrified cells usable for electron tomography

Modern electron microscopy in structural biology on the cellular and molecular level is performed by cryo-electron microscopy and cryo-electron tomography. Cryo-electron tomography (cryo-ET) is the only method to address pleiomorphic structures like cells and organelles in a close to native state, while cryo-electron microscopy (cryo-EM) is applied to study single particles, primarily larger macromolecular complexes, which have been isolated and purified by biochemical methods. Both methodologies provide information on the cellular and molecular level and are therefore ideal for in-depth structural-functional analysis in combination with state of the art biochemical characterisation.

High-end electron microscope for high-resolution cryoEM and cryo-tomography FEI Titan Krios (80 - 300 kV) equipped with an energy filter and a direct detector camera, Phase plate, Falcon 3EC and GIF

Modern electron microscopy in structural biology on the cellular and molecular level is performed by cryo-electron microscopy and cryo-electron tomography. Cryo-electron tomography (cryo-ET) is the only method to address pleiomorphic structures like cells and organelles in a close to native state, while cryo-electron microscopy (cryo-EM) is applied to study single particles, primarily larger macromolecular complexes, which have been isolated and purified by biochemical methods. Both methodologies provide information on the cellular and molecular level and are therefore ideal for in-depth structural-functional analysis in combination with state of the art biochemical characterisation.

Vitrification robot Vitrobot Mark IV

Modern electron microscopy in structural biology on the cellular and molecular level is performed by cryo-electron microscopy and cryo-electron tomography. Cryo-electron tomography (cryo-ET) is the only method to address pleiomorphic structures like cells and organelles in a close to native state, while cryo-electron microscopy (cryo-EM) is applied to study single particles, primarily larger macromolecular complexes, which have been isolated and purified by biochemical methods. Both methodologies provide information on the cellular and molecular level and are therefore ideal for in-depth structural-functional analysis in combination with state of the art biochemical characterisation.

Complete system for glass-slide based microarrays (oligonucleotide, protein, etc.) including scanner, hybridization oven and basic software.

Core Facility Genomics (CFG) offers genomic analysis service and access to up-to-date genomic instrumentation. Main technologies available at the facility are massively parallel sequencing (Illumina), single molecule nanopore sequencing, microarrays and high-throughput & digital PCR. Through cooperation with CEITEC Center of Molecular Medicine it is possible to access other instrumentation – cell sorter, flow cytometers or Laser Capture Microdissection.

8-capillary automatic capillary sequencer AppliedBiosystems Genetic Analyzer 3500 for Sanger sequencing and fragment analysis of nucleic acids. 

Core Facility Genomics (CFG) offers genomic analysis service and access to up-to-date genomic instrumentation. Main technologies available at the facility are massively parallel sequencing (Illumina), single molecule nanopore sequencing, microarrays and high-throughput & digital PCR. Through cooperation with CEITEC Center of Molecular Medicine it is possible to access other instrumentation – cell sorter, flow cytometers or Laser Capture Microdissection.

The Scan^R screening platform can be used for high-content screening using multiwell plates as well as single slides, Petri dishes, and custom-built plates. The software can be used for live cell imaging as well as for imaging of fixed materials. It has fully automated image acquisition which allows the user to select the number of images per well, the number of wells and the different filter sets. Results can be analysed with the image analysis software for automated object detection and cell separation using histograms and gating settings to include or exclude data sets. The system acquires samples in light field and various fluorescence spectra (DAPI, FITC, Cy3, Cy5, and Cy7). This system is not determined for real time acquisition. 

Core Facility Genomics (CFG) offers genomic analysis service and access to up-to-date genomic instrumentation. Main technologies available at the facility are massively parallel sequencing (Illumina), single molecule nanopore sequencing, microarrays and high-throughput & digital PCR. Through cooperation with CEITEC Center of Molecular Medicine it is possible to access other instrumentation – cell sorter, flow cytometers or Laser Capture Microdissection.