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Automated high throughput protein crystal drop imaging system provides ultraviolet and visible crystal imaging and protein crystal monitoring that automatically image crystallization experiments and link images with crystallization conditions.Automated system contains fully integrated system for temperature controlled incubation, inspection, and analysis of protein crystallization plates.

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

High throughput protein crystallisation robotics with pipettors in microlitre and nanolitre volumes, robotics for manipulation with multiwell plates and needed SW and HW.

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

Multichannel (36) SPR biosensor systém for affinity studies of biomolecules, flow-through design, parallel analysis of samples

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

The Monolith system measure equilibrium binding constants for a variety of molecules – thus allows to measure wide range of interactions from ion fragment binding up to interactions of large complexes (liposomes and ribosomes).

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

Dynamic light scattering is used for the analysis of protein solutions, aggregates, promiscuous inhibitors, buffers, nanoparticles, polymers or other products in solution. DLS system measures the polydispersity of the sample and hydrodynamic radii (size) of the particles within range of 0.4 to 5,000 nm. This is particularly beneficial in sample characterization prior to crystallization or other experiments. DynaPro Plate reader allows to measure 96/384 samples in one run.

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

older system purchased before the CEITEC establishment

ProteomeLab XL-I analytical ultracentrifuge is equipped with absorbance (wavelength range 190 – 800 nm) and interference optics and can be used for both sedimentation velocity and sedimentation equilibrium experiments. Analytical ultracentrifugation is applicable for proteins, nucleic acids, lipids, polysaccharides, viruses and nanoparticles. It has a broad applicability including determination of sample homogeneity, oligomeric state of proteins (or molecular weight, respectively) and can be used to assess aggregation of sample and to study biomolecular interactions of self- and hetero-association systems (determination of stoichiometry and affinity).

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

older system purchased before the CEITEC establishment

VP-ITC is isothermal calorimeter with 1400 µl volume of working cell. ITC method is used for characterization of biomolecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and others. Complete thermodynamic profile of the molecular interaction can be obtained in a single experiment (stoichiometry, Ka, ∆H and ∆S).

The core facility provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions.  It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).

older system purchased before the CEITEC establishment

Upright microscope Zeiss AxioImager.Z2Light source: LED white light SOLA.Filters for fluorescence: DAPI, GFP, Cy3.Camera: Hamamatsu ORCA Flash 4.0, sCMOS sensor, 2048 x 2048 pixels, pixel size 6.5 x 6.5 um.Objectives: 20x/0.4 LD Plan Neofluar DIC; 25x/0.8 DIC; 40x/1.3 Plan Apochromat OIL; 40x/1.2 C-Apochromat W; 63x/1.4 Plan Apochromat OIL; 100x/1.4 Plan Apochromat OILSoftware: Visitrone visiview.

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.

Upright microscope Zeiss AxioImager.Z2Light source: LED white light SOLA.Filters for fluorescence: DAPI (F46-000); CFP (F36-544); GFP (F36-525); YFP (F26-303); RFP (F20-451); Cy3(F36-542); Cy5 (F36-523); ChlorophylLP (F46-600).Camera: Hamamatsu ORCA Flash 4.0 LT, sCMOS sensor, 2048 x 2048 pixels, pixel size 6.5 x 6.5 um.Objectives: LD PlanNeofluar 20x/0.4; LD LCI Plan Apo 25x/0.8 multi immersion; Plan Apochromat 40x/1.2 OIL; Plan Apochromat 63x/1.4 OIL; Plan Apochromat 100x/1.4 OIL; C Apochromat 40x/1.2 W.Software: Visitrone visiview.

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.

Inverted microscope Zeiss Axio Observer with laser scanning unit LSM 880. Microscope disposes solid state lasers - 405, 561, 594 and 633 nm and argon laser - 458, 488, 514 nm. For detection of fluorescence signal in confocal mode there are two PMT detectors, 32 channel spectral GaAsP detector and Airyscan Fast detector and T-PMT for transmission light detection. Lasers: 405, 458, 488, 514, 561, 594, 640 nm. Detectors: 2 x PMT detectors, 1 x GaAsp PMT spectral detector, AiryscanFast detector, T-PMT detector. Filters for Airyscan: BP420-480 + LP 605, BP465-505 + LP 525, BP495-550 + LP 570, BP555-620 + LP 645, BP420-480 + BP 495-550, BP420-445 + BP 465-505. Objectives: 10x/0.3 M27 EC Plan-Neofluar; 20x/0.5 M27 EC Plan-Neofluar; 25x/0.8 M27 LD LCI Plan-Apochromat W, Oil, Glycerol korr; 40x/1.2 M27 W Korr C-Apochromat; 63x/1.3 M27 Imm korr LCI Plan-Neofluar; 63x/1.4 M27 OIL DIC Plan-Apochromat, 100x/1.46 M27 OIL DIC Plan-Apochromat. Software: ZEN black. 

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.